Synonym
SP7&NSP8; nsp7&nsp8
Source / expression
SARS-COV-2 NSP7&NSP8 Protein is expressed from E.coil with His tag at the C-Terminus.It contains Ser1-Gln83(NSP7)&Ala1-Gln198(NSP8) [Accession | YP_009725303.1(NSP7)&YP_009725304.1(NSP8)].
Molecular weight
The protein has a predicted MW of 32.8 kDa same as Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM Nacl, 200mM Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
