Synonym
3CL Protease; 3CL-Mpro; M Proteinase; Mpro
Source / expression
Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein (P132H) is expressed from E.coli without tag.It contains Ser1-Gln306(P132H) [Accession | YP_009725301.1].
Molecular weight
The protein has a predicted MW of 33.8 kDa same as Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Supplied as 0.22μm filtered solution in 25mM HEPES, 2.5mM DTT, 10% Glycerol (pH 7.5).
Storage
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.