SARS-COV-2 Spike S Trimer (D614G) Protein

Name: SARS-COV-2 Spike S Trimer (D614G) Protein
Brand: Puweituo Bio
SKU: PR499101

Cat. No.PR499101

Key advantages

High purity

Expression host / system: HEK293
Species: —
Tags: —
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Activity: Contact for data package

Representative QC

SARS-COV-2 Spike S Trimer (D614G) Protein

Technical specifications

Synonym

S protein; Spike glycoprotein; S glycoprotein; COVID-19

Source / expression

Recombinant SARS-COV-2 Spike S Trimer(D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188 (D614G) trimer design [Accession | QHD43416.1].

Molecular weight

The protein has a predicted MW of 136.7 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Bis-Tris PAGE result.

Endotoxin

Less than 1 EU per μg by the LAL method.

Purity

> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Storage

-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

References

(1)Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, Kyratsous CA, Dudkina NV, Shen K, Luban J. Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant. Cell. 2020 Oct 29;183(3):739-751.e8. doi: 10.1016/j.cell.2020.09.032 Epub 2020 Sep 15. PMID: 32991842; PMCID: PMC7492024.

Background

The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.

FOLR1-PH1101

Non-biotinylated Human FOLR1 Protein

SARS-COV-2 Spike S Trimer (D614G) Protein

Technical specifications

References

Background

Related products

Services & discovery

SARS-COV-2 Spike S Trimer (D614G) Protein

Technical specifications

References

Background

Related products

Services & discovery