Synonym
SIRP-beta-1 isoform 3; SIRPB1; CD172b; DKFZp686A05192; FLJ26614; 9930027N05Rik; SIRP-beta; Sirpb; Sirpb1; Sirpb1a;SIRP-β-1 isoform 3; SIRP-β
Source / expression
Recombinant Human SIRP Beta 1 isoform 3 Proteinis expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu30-Leu371 [Accession | Q5TFQ8-1].
Molecular weight
The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 0.1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
