Synonym
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9
Source / expression
Recombinant Human 4-1BB Ligand/TNFSF9 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Arg71-Glu254 trimer design [Accession | P41273].
Molecular weight
The protein has a predicted MW of 61.8 kDa same as Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in PBS, 300mM NaCL (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
