Synonym
Integrin alpha 10 beta 1; ITGA10&ITGB1; α10β1
Source / expression
Recombinant Human Integrin alpha 10 beta 1 (ITGA10&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of ITGA10.It contains Phe23-Ser1122(ITGA10) acidic tail and Gln21-Asp728(ITGB1) basic tail [Accession | O75578-1(ITGA10)&P05556-1(ITGB1)].
Molecular weight
The protein has a predicted MW of 125.60 kDa (ITGA10) & 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGA10) and 110-120 kDa (ITGB1) based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
