Synonym
ITGA8&ITGB1; ; α8β1; Integrin alpha 8 beta 1;
Source / expression
Recombinant Human Integrin alpha 8 beta 1 (ITGA8&ITGB1) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of ITGA8.It contains Phe39-Leu1012(ITGA8) acidic tail and Gln21-Asp728(ITGB1) basic tail [Accession | P53708(ITGA8)&P05556-1(ITGB1)].
Molecular weight
The protein has a predicted MW of 113.90 kDa (ITGA8) & 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 120-140 kDa (ITGA8) and 100-120 kDa (ITGB1) based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
