Synonym
BLAST-2; CD23; CD23A; CLEC4J; FCE2; IGEBF; FCER2; FcεRII; Fcr; Fcgr; Fc receptor; CLEC-6; CLECSF8; MCL; MGC40078; MPCL; Fcer2a; Ly-42
Source / expression
Recombinant Biotinylated Human CD23/Fc epsilon RII Protein is expressed from HEK293 with His tag and Avi tag at the N-terminus.It contains Asp48-Ser321 [Accession | P06734-1].
Molecular weight
The protein has a predicted MW of 33.89 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
