Synonym
Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A; FcγRIIA; Fc γRIIA; Fcγ RIIA; Fc γ RIIA
Source / expression
Recombinant Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln34-Ile218(R167) [Accession | P12318-1].
Molecular weight
The protein has a predicted MW of 23.4 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
