Synonym
CD314; D12S2489E; KLR; NKG2-D; NKG2D
Source / expression
Recombinant Biotinylated Human NKG2D/CD314 Protein is expressed from HEK293 with Avi tag and His tag at the N-Terminus.It contains Phe78-Val216 [Accession | P26718].
Molecular weight
The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
