Synonym
GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Source / expression
Recombinant Biotinylated Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln25-His559 [Accession | P51654-1].
Molecular weight
The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 30-31 kDa, 38-42 kDa and 65-80 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
