Synonym
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Source / expression
Recombinant Human FAP Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Leu26-Asp760 [Accession | Q12884-1].
Molecular weight
The protein has a predicted MW of 112.3 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 0.1 EU per μg by the LAL method.
Purity
> 90% as determined by Bis-Tris PAGE
> 90% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.