Synonym
CEDLAP; DPD1; TGFB1; TGF-β1; TGF-beta 1
Source / expression
Recombinant Human Mature TGF beta 1 Protein is expressed from HEK293 without tag.It contains Ala279-Ser390 [Accession | P01137].
Molecular weight
The protein has a predicted MW of 13.2 kDa (monomer). Due to glycosylation, the protein migrates to 14-15 kDa (monomer) under reduced (R) condition and 26-30 kDa (dimer) under Non reduced (N) condition based on Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
Formulation
Lyophilized from 0.22μm filtered solution in 50mM Glycine 150mM NaCl (pH 2.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 4mM HCL. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
