Synonym
VEGF; VEGFA; L-VEGF; N-VEGF; VPF; MVCD1
Source / expression
Recombinant Human VEGF110 Protein is expressed from HEK293 without tag.It contains Ala27-Arg136 [Accession | P15692-9].
Molecular weight
The protein has a predicted MW of 12.70 kDa. Due to glycosylation, the protein migrates to 13 kDa and 17-23 kDa based on Bis-Tris PAGE result.
Endotoxin
Less than 0.1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
