Synonym
Interleukin-21; IL-21; Za11; CVID11
Source / expression
Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162 [Accession | Q9HBE4-1].
Molecular weight
The protein has a predicted MW of 15.4 kDa same as Bis-Tris PAGE result.
Endotoxin
Less than 1 EU per μg by the LAL method.
Purity
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Formulation
Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 20mM NaAc (pH 5.5). Please refer to the Certificate of Analysis for detailed instructions.
Storage
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.