Biotinylated Human PDGF R alpha/PDGFRA Protein

Name: Biotinylated Human PDGF R alpha/PDGFRA Protein
Brand: Puweituo Bio
SKU: PDGFRA-HB001

Catalog numberPDGFRA-HB001

HumanHEK293Molecule · PDGFRAMolecular weight · 59.2 kDa

Product introduction

Recombinant Biotinylated Human PDGF R alpha/PDGFRA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln24-Glu524 (UniProt accession: P16234).

Product details

Molecular aliases: PDGFRA; CD140A; PDGFR2; RHEPDGFRA; MGC74795; PDGFR-2
Protein length: Gln24-Glu524
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human PDGF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human PDGF R alpha is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human PDGF R alpha, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test).

Background

Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.

References

1.Shi Y, He G, Lee WC, McKenzie JA, Silva MJ, Long F. Gli1 identifies osteogenic progenitors for bone formation and fracture repair. Nat Commun. 2017;8(1):2043. Published 2017 Dec 11. doi:10.1038/s41467-017-02171-2

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Biotinylated Human PDGF R alpha/PDGFRA Protein

Catalog numberPDGFRA-HB001

HumanHEK293Molecule · PDGFRAMolecular weight · 59.2 kDa

Biotinylated Human PDGF R alpha/PDGFRA Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human PDGF R alpha/PDGFRA Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References