Biotinylated Human NPR1/NPRA Protein (active dimer)

Name: Biotinylated Human NPR1/NPRA Protein (active dimer)
Brand: Puweituo Bio
SKU: NPR1-HB001

Catalog numberNPR1-HB001

HumanHEK293Molecule · ANP-AMolecular weight · 51.8 kDa

Product introduction

Recombinant Biotinylated Human NPR1/NPRA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly33-Glu473 (accession: NP_000897.3).

Product details

Molecular aliases: ANP-A; ANPR-A; NPR-A; GC-A; ANPRA
Protein length: Gly33-Glu473
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human NPR1 is greater than 90% as determined by SEC-HPLC.

SEC-MALS Data — The purity of Biotinylated Human NPR1 is greater than 90% and the molecular weight of this protein is around 110-120 kDa as determined by SEC-MALS.

ELISA Data — Immobilized Biotinylated Human NPR1, His-Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).

SPR Data — Anti-NPR1 Ab.2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NPR1, His Tag with an affinity constant of 2.34 µM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human NPR1, His-Avi tag on Streptavidin-Biosensor can bind Anti-NPR1 Ab.2, hFc Tag with an affinity constant of 1.35 nM as determined in BLI assay.

Background

NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.

References

1.Tokudome T, Otani K, Mao Y, Jensen LJ, Arai Y, Miyazaki T, Sonobe T, Pearson JT, Osaki T, Minamino N, Ishida J, Fukamizu A, Kawakami H, Onozuka D, Nishimura K, Miyazato M, Nishimura H. Endothelial Natriuretic Peptide Receptor 1 Play Crucial Role for Acute and Chronic Blood Pressure Regulation by Atrial Natriuretic Peptide. Hypertension. 2022 Jul;79(7):1409-1422. doi: 10.1161/HYPERTENSIONAHA.121.18114. Epub 2022 May 9. PMID: 35534926.

加载中…

Biotinylated Human NPR1/NPRA Protein (active dimer)

Catalog numberNPR1-HB001

HumanHEK293Molecule · ANP-AMolecular weight · 51.8 kDa

Product introduction

Recombinant Biotinylated Human NPR1/NPRA Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gly33-Glu473 (accession: NP_000897.3).

Biotinylated Human NPR1/NPRA Protein (active dimer)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References

Biotinylated Human NPR1/NPRA Protein (active dimer)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SEC-MALS

SEC-MALS Data

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References