FITC-Labeled Human MSLN/Mesothelin Protein

Name: FITC-Labeled Human MSLN/Mesothelin Protein
Brand: Puweituo Bio
SKU: MSLN-HP002

Catalog numberMSLN-HP002

HumanHEK293Molecule · MSLNMolecular weight · 59 kDa

Product introduction

Recombinant FITC-Labeled Human MSLN/Mesothelin Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Glu296-Gly580 (UniProt accession: Q13421-2).

Product details

Molecular aliases: MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
Protein length: Glu296-Gly580
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Formulation: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — FITC-Labeled Human MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of FITC-Labeled Human MSLN is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized FITC-Labeled Human MSLN, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 7.9ng/ml determined by ELISA (QC Test).

Flow Cytometry Data — FACS Analysis of Anti-MSLN CAR Expression. 293T cells were transfected with anti-MSLN-scFv and Fc tag. Cells were stained with 5μg/ml FITC-Labeled Human MSLN (296-580), Fc Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

Background

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

References

1.Adusumilli P S, Cherkassky L, Villena-Vargas J, et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.
2.Morello A, Sadelain M, Adusumilli P S. Mesothelin-Targeted CARs: Driving T cells to Solid Tumors[J]. Cancer Discovery, 2015, 6(2).

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FITC-Labeled Human MSLN/Mesothelin Protein

Catalog numberMSLN-HP002

HumanHEK293Molecule · MSLNMolecular weight · 59 kDa

FITC-Labeled Human MSLN/Mesothelin Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Flow Cytometry

Flow Cytometry Data

Background

References

FITC-Labeled Human MSLN/Mesothelin Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Flow Cytometry

Flow Cytometry Data

Background

References