Biotinylated Human MMP-9 Protein (pro form)

Name: Biotinylated Human MMP-9 Protein (pro form)
Brand: Puweituo Bio
SKU: MMP9-HB001

Catalog numberMMP9-HB001

HumanHEK293Molecule · MMP-9Molecular weight · 79.3 kDa

Product introduction

Recombinant Biotinylated Human MMP-9 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. The protein needs to be activated by APMA for an activated form. It contains amino acid residues Ala20-Asp707 (UniProt accession: P14780).

Product details

Molecular aliases: MMP-9; GELB; CLG4B; MANDP2; Gelatinase B; MMP9
Protein length: Ala20-Asp707
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 95-105 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 10mM CaCl 2, 150mM NaCl, 0.05% Brij-35 (w/v) (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human MMP-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human MMP-9 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human MMP-9, His Avi tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MMP-9 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. (QC Test)

Background

Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.

References

1.Zhang C, Lim J, Jeon HH, et al. FOXO1 deletion in keratinocytes improves diabetic wound healing through MMP9 regulation. Sci Rep. 2017;7(1):10565. Published 2017 Sep 5. doi:10.1038/s41598-017-10999-3

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Biotinylated Human MMP-9 Protein (pro form)

Catalog numberMMP9-HB001

HumanHEK293Molecule · MMP-9Molecular weight · 79.3 kDa

Biotinylated Human MMP-9 Protein (pro form)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human MMP-9 Protein (pro form)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References