Biotinylated Human MASP3 Protein

Name: Biotinylated Human MASP3 Protein
Brand: Puweituo Bio
SKU: MASP3-HB001

Catalog numberMASP3-HB001

HumanHEK293Molecule · MASP-3Molecular weight · 33.46 kDa

Product introduction

Recombinant Biotinylated Human MASP3 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile450-Arg728 (UniProt accession: P48740-2).

Product details

Molecular aliases: MASP-3; MASP-1; MASP1; RaRF; Serine protease 5; CRARF; CRARF1; PRSS5;; MASP3
Protein length: Ile450-Arg728
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 33.46 kDa. Due to glycosylation, the protein migrates to 42-55 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human MASP3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human MASP3 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human MASP3, His Avi tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. (QC Test)

Background

Mannose-binding lectin-associated serine protease-1 (also named MASP3), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system.

References

1.Mu L, Yin X, Wu H, Han K, Wu L, Ding M, Bian X, Li B, Fu S, Liang F, Guo Z, Ye J. Expression and functional characterization of a mannose-binding lectin-associated serine protease-1 (MASP-1) from Nile tilapia (Oreochromis niloticus) in host defense against bacterial infection. Fish Shellfish Immunol. 2019 Aug;91:68-77. doi: 10.1016/j.fsi.2019.05.014. Epub 2019 May 13. PMID: 31096060.

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Biotinylated Human MASP3 Protein

Catalog numberMASP3-HB001

HumanHEK293Molecule · MASP-3Molecular weight · 33.46 kDa

Product introduction

Recombinant Biotinylated Human MASP3 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile450-Arg728 (UniProt accession: P48740-2).

Biotinylated Human MASP3 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human MASP3 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References