Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein
Brand: Puweituo Bio
SKU: MART1-HB002

Catalog numberMART1-HB002

HumanHEK293Molecule · MART1Molecular weight · 50.40 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and ELAGIGILTV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &ELAGIGILTV).

Product details

Molecular aliases: MART1; LB39-AA; SK29-AA; Melan-A; MLANA
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.40 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi tag at 0.5μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9ng/ml determined by ELISA.

Background

Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.

References

1.Wang J, Jia R, Yao Y, Cun B, Huang X, Gu P, Ge S, Fan X. Differential expression of Mart-1 in human uveal melanoma cells. Mol Med Rep. 2011 Sep-Oct;4(5):799-803. doi: 10.3892/mmr.2011.504. Epub 2011 Jun 7. PMID: 21667026.

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Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein

Catalog numberMART1-HB002

HumanHEK293Molecule · MART1Molecular weight · 50.40 kDa

Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References