Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein, Ultra Low Endotoxin

Name: Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein, Ultra Low Endotoxin
Brand: Puweituo Bio
SKU: MAGEA4-HB001

Catalog numberMAGEA4-HB001

HumanHEK293Molecule · HLA0201Molecular weight · 50.5 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and GVYDGREHTV peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &GVYDGREHTV).

Product details

Molecular aliases: HLA0201; MHC I; MAGE-A4; CT1.4; MAGE4A; MAGE4B; MAGE-X2; member 4
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.01 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer was greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.

Background

Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene. The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.

References

1.(1)Kageyama S, Ikeda H, Miyahara Y, et al. Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer[J]. Clinical Cancer Research, 2015, 21(10):2268.

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Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein, Ultra Low Endotoxin

Catalog numberMAGEA4-HB001

HumanHEK293Molecule · HLA0201Molecular weight · 50.5 kDa

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References