Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein

Name: Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein
Brand: Puweituo Bio
SKU: MAGEA3-HB002

Catalog numberMAGEA3-HB002

HumanHEK293Molecule · Melanoma-associated antigen 3Molecular weight · 50.30 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*01:01), Ile21-Met119(B2M) and EVDPIGHLY peptide (accession: Q5SUL5(HLA-A*01:01) & P61769(B2M) &EVDPIGHLY).

Product details

Molecular aliases: Melanoma-associated antigen 3; MZ2-D; MZ2D; MZ2-D; CT1.3; MAGE-3
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer, His Tag at 1μg/ml(100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.9ng/ml determined by ELISA (QC Test).

Background

Melanoma antigen gene A3 (MAGE-A3) is one of the most immunogenic cancer testis antigens and is common in various types of cancers. MAGE-A3 can be considered as a predictor for poor prognosis and an option for vaccine immunotherapy in patients with PCa.

References

1.Khalvandi A, Abolhasani M, Madjd Z, Shekarabi M, Kourosh-Arami M, Mohsenzadegan M. Nuclear overexpression levels of MAGE-A3 predict poor prognosis in patients with prostate cancer. APMIS. 2021 Jun;129(6):291-303. doi: 10.1111/apm.13132. Epub 2021 Apr 13. PMID: 33743542.

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Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein

Catalog numberMAGEA3-HB002

HumanHEK293Molecule · Melanoma-associated antigen 3Molecular weight · 50.30 kDa

Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*01:01&B2M&MAGE-A3 (EVDPIGHLY) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References