Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein
Brand: Puweituo Bio
SKU: MAGEA2-HB001

Catalog numberMAGEA2-HB001

HumanHEK293Molecule · MAGE-A2;MAGEA2Molecular weight · 50.50 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KMVELVHFL peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) &KMVELVHFL).

Product details

Molecular aliases: MAGE-A2;MAGEA2
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer is greater than 95% as determined by SEC-HPLC.

Background

Melanoma antigen gene (MAGE)-A2 (MAGEA2) is a member of the MAGE-A family proteins widely studied for cancer vaccine development and identification of tumor markers. MAGEA2 plays an oncogenic role in glioma progression, and they provide insight into MAGEA2 application as a novel predictor of clinical outcomes and a potential glioma biomarker.

References

1.Meng Q, Luo G, Liu B, Sun Y, Yan Z. Melanoma-associated antigen A2 is overexpressed in glioma and associated with poor prognosis in glioma patients. Neoplasma. 2018;65(4):604-609. doi: 10.4149/neo_2018_170625N440. PMID: 30064232.

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Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein

Catalog numberMAGEA2-HB001

HumanHEK293Molecule · MAGE-A2;MAGEA2Molecular weight · 50.50 kDa

Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&MAGE-A2 (KMVELVHFL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References