Biotinylated Cynomolgus LY6G6D Protein

Name: Biotinylated Cynomolgus LY6G6D Protein
Brand: Puweituo Bio
SKU: LY6G6D-CB001

Catalog numberLY6G6D-CB001

HEK293Molecule · Protein Ly6-DMolecular weight · 12.03 kDa

Product introduction

Recombinant Biotinylated Cynomolgus LY6G6D Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Asn20-Ser104 (accession: XP_014991478.1).

Product details

Molecular aliases: Protein Ly6-D; C6orf23; G6D; MEGT1; NG25; Protein Ly6D
Protein length: Asn20-Ser104
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 12.03 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus LY6G6D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Cynomolgus LY6G6D is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Cynomolgus LY6G6D, His-Avi tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-LY6G6D Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. (QC Test)

BLI Data — Loaded Anti-LY6G6D Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Cynomolgus LY6G6D, His-Avi tag with an affinity constant of 1.55 nM as determined in BLI assay.

Background

LY6G6D is a selectively expressed colorectal cancer antigen that can be used for targeting a therapeutic T-cell response by a T-cell engager. LY6G6D was identified as a selectively expressed CRC antigen that can be utilized to potently re-direct and activate cytotoxic T-cells to lyse LY6G6D expressing CRC using a TcE. This effect can be spread to target negative neighboring tumor cells, potentially leading to improved therapeutic efficacy.

References

1.Corrales L, Hipp S, Martin K, Sabarth N, Tirapu I, Fuchs K, Thaler B, Walterskirchen C, Bauer K, Fabits M, Bergmann M, Binder C, Chetta PM, Vogt AB, Adam PJ. LY6G6D is a selectively expressed colorectal cancer antigen that can be used for targeting a therapeutic T-cell response by a T-cell engager. Front Immunol. 2022 Sep 8;13:1008764. doi: 10.3389/fimmu.2022.1008764. PMID: 36159851; PMCID: PMC9493073.

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Biotinylated Cynomolgus LY6G6D Protein

Catalog numberLY6G6D-CB001

HEK293Molecule · Protein Ly6-DMolecular weight · 12.03 kDa

Product introduction

Recombinant Biotinylated Cynomolgus LY6G6D Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Asn20-Ser104 (accession: XP_014991478.1).

Biotinylated Cynomolgus LY6G6D Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

Background

References

Biotinylated Cynomolgus LY6G6D Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

BLI

BLI Data

Background

References