Human LTBR Protein

Name: Human LTBR Protein
Brand: Puweituo Bio
SKU: LTBR-HF001

Catalog numberLTBR-HF001

HumanHEK293Molecule · TNFCRMolecular weight · 48.5 kDa

Product introduction

Recombinant Human LTBR Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gln31-Met227 (UniProt accession: P36941-1).

Product details

Molecular aliases: TNFCR; TNFR3; TNFRSF3; LTBR; CD18; TNFR2-RP; TNFR-RP; LT-BETA-R; TNF-R-III; D12S370; LT-β-R;TNFR2RP;LT-b-R
Protein length: Gln31-Met227
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human LTBR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Human LIGHT, His Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 13.8 ng/ml determined by ELISA (QC Test).

Background

Components of lymphotoxin beta receptor (LTBR)-associated signaling complexes, including TRAF2, TRAF3, NIK, IKK1, and IKK2 have been shown to participate in the coupling of LTBR to NFkappaB. TRAF3 functions as a negative regulator of LTBR signaling via both canonical and non-canonical NFkappaB pathways by two distinct mechanisms. These effects of TRAF3 depletion did not require LTBR signaling and were consistent with autonomous activation of the non-canonical NFkappaB pathway.

References

1.Bista P, Zeng W, Ryan S, Bailly V, Browning JL, Lukashev ME. TRAF3 controls activation of the canonical and alternative NFkappaB by the lymphotoxin beta receptor. J Biol Chem. 2010 Apr 23;285(17):12971-8. doi: 10.1074/jbc.M109.076091. Epub 2010 Feb
2.PMID: 20185819; PMCID: PMC2857099.

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Human LTBR Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

Background

References

Human LTBR Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

ELISA

ELISA Data

Background

References