Biotinylated Human LPAR1 Nanodisc

Name: Biotinylated Human LPAR1 Nanodisc
Brand: Puweituo Bio
SKU: LPAR1-HB001

Catalog numberLPAR1-HB001

HumanHEK293Molecule · LPA receptor 1Molecular weight · 54.10 kDa

Product introduction

Recombinant Biotinylated Human LPAR1 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val364 (UniProt accession: Q92633-1).

Product details

Molecular aliases: LPA receptor 1; LPA-1; EDG2; LPA1; Gpcr26
Protein length: Met1-Val364
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 54.10 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human LPAR1 Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LPAR1 Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. (QC Test)

SPR Data — Biotinylated Human LPAR1 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-LPAR1 Antibody, hFc Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200).

Background

Lysophosphatidic acid receptor 1 (LPA1) belongs to the G protein-coupled receptor family. The ligand for LPA1 is LPA, the simplest lysophospholipid. The mature form of LPA1 is glycosylated and localized on the plasma membrane. LPA1 is bound to heterotrimetric G proteins and transduces intracellular signaling in response to ligation to LPA. Desensitization of LPA1 negatively regulates LPA1-mediated signaling and the resulting biological functions.

References

1.Zhao J, Stephens T, Zhao Y. Molecular Regulation of Lysophosphatidic Acid Receptor 1 Maturation and Desensitization. Cell Biochem Biophys. 2021 Sep;79(3):477-483. doi: 10.1007/s12013-021-00999-6. Epub 2021 May 25. PMID: 34032994; PMCID: PMC8887818.

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Biotinylated Human LPAR1 Nanodisc

Catalog numberLPAR1-HB001

HumanHEK293Molecule · LPA receptor 1Molecular weight · 54.10 kDa

Product introduction

Recombinant Biotinylated Human LPAR1 Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Val364 (UniProt accession: Q92633-1).

Biotinylated Human LPAR1 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human LPAR1 Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

Background

References