Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer Protein

Name: Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer Protein
Brand: Puweituo Bio
SKU: KRASWT-HP006

Catalog numberKRASWT-HP006

HumanE. coliMolecule · MHCMolecular weight · 35.36 kDa

Product introduction

Recombinant Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVGAGGVGK peptide (accession: AAV53343.1(HLA-A*11:01) & P61769(B2M) &VVGAGGVGK).

Product details

Molecular aliases: MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
Protein length: Gly25-Thr305
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).

Background

Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target.

References

1.(1) Wu HZ, Xiao JQ, Xiao SS, Cheng Y. KRAS: A Promising Therapeutic Target for Cancer Treatment. Curr Top Med Chem. 2019;19(23):2081-2097. doi: 10.2174/1568026619666190905164144. PMID: 31486755.

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Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer Protein

Catalog numberKRASWT-HP006

HumanE. coliMolecule · MHCMolecular weight · 35.36 kDa

Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human HLA-A*11:01&B2M&KRAS WT (VVGAGGVGK) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References