Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein, Ultra Low Endotoxin

Name: Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein, Ultra Low Endotoxin
Brand: Puweituo Bio
SKU: KRASG12V-HB008

Catalog numberKRASG12V-HB008

HumanHEK293Molecule · MHCMolecular weight · 50.3 kDa

Product introduction

Recombinant Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide (accession: AAV53343.1(HLA-A*11:01) & P61769(B2M) &VVVGAVGVGK).

Product details

Molecular aliases: MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.01 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 10.7 ng/ml determined by ELISA (QC Test).

Background

Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.

References

1.(1) Wu HZ, Xiao JQ, Xiao SS, Cheng Y. KRAS: A Promising Therapeutic Target for Cancer Treatment. Curr Top Med Chem. 2019;19(23):2081-2097. doi: 10.2174/1568026619666190905164144. PMID: 31486755.

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Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein, Ultra Low Endotoxin

Catalog numberKRASG12V-HB008

HumanHEK293Molecule · MHCMolecular weight · 50.3 kDa

Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References