Biotinylated Human HLA-C*08:01&B2M&KRAS G12D (GADGVGKSAL) Monomer Protein

Name: Biotinylated Human HLA-C*08:01&B2M&KRAS G12D (GADGVGKSAL) Monomer Protein
Brand: Puweituo Bio
SKU: KRASG12D-HB008

Catalog numberKRASG12D-HB008

HumanHEK293Molecule · MHCMolecular weight · 50.60 kDa

Product introduction

Recombinant Biotinylated Human HLA-C*08:01&B2M&KRAS G12D (GADGVGKSAL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Cys25-Ala366(HLA-C*08:01), Ile21-Met119(B2M) and GADGVGKSAL peptide (accession: AAA59688.1(HLA-C*08:01) & P61769(B2M) &GADGVGKSAL).

Product details

Molecular aliases: MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
Protein length: Cys25-Ala366
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-C*08:01&B2M&KRAS G12D (GADGVGKSAL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-C*08:01&B2M&KRAS G12D (GADGVGKSAL) Monomer is greater than 95% as determined by SEC-HPLC.

Background

Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.

References

1.(1) Wu HZ, Xiao JQ, Xiao SS, Cheng Y. KRAS: A Promising Therapeutic Target for Cancer Treatment. Curr Top Med Chem. 2019;19(23):2081-2097. doi: 10.2174/1568026619666190905164144. PMID: 31486755.

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Biotinylated Human HLA-C*08:01&B2M&KRAS G12D (GADGVGKSAL) Monomer Protein

Catalog numberKRASG12D-HB008

HumanHEK293Molecule · MHCMolecular weight · 50.60 kDa

Biotinylated Human HLA-C*08:01&B2M&KRAS G12D (GADGVGKSAL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human HLA-C*08:01&B2M&KRAS G12D (GADGVGKSAL) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References