Human IL-1 alpha/IL-1A Protein

Name: Human IL-1 alpha/IL-1A Protein
Brand: Puweituo Bio
SKU: IL1A-HP001

Catalog numberIL1A-HP001

HumanE. coliMolecule · Interleukin-1 alphaMolecular weight · 18 kDa

Product introduction

Recombinant Human IL-1 alpha/IL-1A Protein is expressed in E. coli without tag. It contains amino acid residues Ser113-Ala271 (accession: NP_000566.3).

Product details

Molecular aliases: Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1
Protein length: Ser113-Ala271
Expression system: E. coli
Molecular weight: The protein has a predicted MW of 18 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.05 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.

SPR Data — Human IL-1R1, His Tag (Cat. IL1-HM1R1) captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). (QC Test)

BLI Data — Loaded Human IL-1R1, His Tag (Cat. IL1-HM1R1) on Anti-His-Biosensor can bind Human IL-1 alpha, No Tag with an affinity constant of 0.84 nM as determined in BLI assay.

Cell Based Assay Data — Measured by a reporter gene assay. The ED50 for this effect is 1-15 pg/mL. (QC Test)

Background

The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.

References

1.Malik A, Kanneganti TD. Function and regulation of IL-1α in inflammatory diseases and cancer. Immunol Rev. 2018 Jan;281(1):124-137. doi: 10.1111/imr.12615. PMID: 29247991; PMCID: PMC5739076.

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Human IL-1 alpha/IL-1A Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

BLI

BLI Data

Bioactivity

Cell Based Assay Data

Background

References

Human IL-1 alpha/IL-1A Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

BLI

BLI Data

Bioactivity

Cell Based Assay Data

Background

References