Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling)

Name: Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: IL15-HB003

Catalog numberIL15-HB003

HumanHEK293Molecule · IL-15 R alphaMolecular weight · 23.8 kDa

Product introduction

Recombinant Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Ile31-Ser108 (IL-15RA)&Asn49-Ser162 (IL-15) (accession: Q13261-1 (IL-15RA) & P40933-1 (IL-15)).

Product details

Molecular aliases: IL-15 R alpha; CD215; IL15RA; IL-15RA; IL-15R-alpha1; Interleukin-15; IL-15; IL15; Nanrilkefusp alfa; IL15R alpha; IL15R-alpha1; IL 15; Interleukin 15
Protein length: Ile31-Ser108
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human IL-15RA&IL-15 (Primary Amine Labeling) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data — Immobilized Biotinylated Human IL-15Ra&IL-15, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.8 ng/ml determined by ELISA.

Background

Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

References

1.Nadeau L, Aguer C. Interleukin-15 as a myokine: mechanistic insight into its effect on skeletal muscle metabolism. Appl Physiol Nutr Metab. 2019 Mar;44(3):229-238. doi: 10.1139/apnm-2018-0022. Epub 2018 Sep 6. PMID: 30189147.

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Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling)

Catalog numberIL15-HB003

HumanHEK293Molecule · IL-15 R alphaMolecular weight · 23.8 kDa

Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References

Biotinylated Human IL-15RA&IL-15 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

ELISA

ELISA Data

Background

References