Biotinylated Human IL-13Ra2 Protein

Name: Biotinylated Human IL-13Ra2 Protein
Brand: Puweituo Bio
SKU: IL13RA2-HB001

Catalog numberIL13RA2-HB001

HumanHEK293Molecule · CD213a2Molecular weight · 40.09 kDa

Product introduction

Recombinant Biotinylated Human IL-13Ra2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp27-Leu342 (UniProt accession: Q14627).

Product details

Molecular aliases: CD213a2; IL-13 R alpha 2; IL13BP; IL13R; IL13RA2; IL-13R subunit alpha-2; IL-13Ra2;IL13R alpha 2; IL13R subunit alpha-2
Protein length: Asp27-Leu342
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 40.09 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human IL-13Ra2 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-13, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra2, His Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test).

Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

References

1.Suzuki A, Leland P, Joshi B H, et al. Targeting of IL-4 and IL-13 receptors for cancer therapy[J]. Cytokine, 2015, 75(1):S1043466615002124.

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Biotinylated Human IL-13Ra2 Protein

Catalog numberIL13RA2-HB001

HumanHEK293Molecule · CD213a2Molecular weight · 40.09 kDa

Product introduction

Recombinant Biotinylated Human IL-13Ra2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Asp27-Leu342 (UniProt accession: Q14627).

Biotinylated Human IL-13Ra2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human IL-13Ra2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References