Human IL-13Ra1 Protein

Name: Human IL-13Ra1 Protein
Brand: Puweituo Bio
SKU: IL13RA1-HF001

Catalog numberIL13RA1-HF001

HumanHEK293Molecule · IL-13 R alpha 1Molecular weight · 63.4 kDa

Product introduction

Recombinant Human IL-13Ra1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly22-Thr343 (UniProt accession: P78552).

Product details

Molecular aliases: IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1; AI882074;IL13R alpha 1
Protein length: Gly22-Thr343
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 63.4 kDa. Due to glycosylation, the protein migrates to 75-110 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human IL-13Ra1 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-13Ra1 is greater than 95% as determined by SEC-HPLC.

SPR Data — Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13, His Tag with an affinity constant of 24.64 nM as determined in SPR assay (Biacore T200). (QC Test)

BLI Data — Loaded Human IL-13, His Tag on Anti-His-Biosensor can bind Human IL-13Ra1, hFc Tag with an affinity constant of 40.70 nM as determined in BLI assay.

Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

References

1.Suzuki A, Leland P, Joshi B H, et al. Targeting of IL-4 and IL-13 receptors for cancer therapy[J]. Cytokine, 2015, 75(1):S1043466615002124. Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.

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Human IL-13Ra1 Protein

Catalog numberIL13RA1-HF001

HumanHEK293Molecule · IL-13 R alpha 1Molecular weight · 63.4 kDa

Product introduction

Recombinant Human IL-13Ra1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Gly22-Thr343 (UniProt accession: P78552).

Human IL-13Ra1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

BLI

BLI Data

Background

References

Human IL-13Ra1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

BLI

BLI Data

Background

References