Biotinylated Human IL-13 Protein

Name: Biotinylated Human IL-13 Protein
Brand: Puweituo Bio
SKU: IL13-HB001

Catalog numberIL13-HB001

HumanHEK293Molecule · IL13Molecular weight · 15.3 kDa

Product introduction

Recombinant Biotinylated Human IL-13 Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Gly21-Asn132 (accession: AAK53823).

Product details

Molecular aliases: IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789; Interleukin-13; IL 13
Protein length: Gly21-Asn132
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated IL-13 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human IL-13 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human IL-13, His-Avi tag at 5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81μg/ml determined by ELISA (QC Test).

SPR Data — Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human IL-13, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).

Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).

References

1.Grünig G, Corry D B, Reibman J, et al. Interleukin 13 and the evolution of asthma therapy[J]. American Journal of Clinical & Experimental Immunology, 2012, 1(1):20.

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Biotinylated Human IL-13 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References

Biotinylated Human IL-13 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Background

References