Human IL-10 Protein

Name: Human IL-10 Protein
Brand: Puweituo Bio
SKU: IL10-HP001

Catalog numberIL10-HP001

HumanHEK293Molecule · Interleukin-10Molecular weight · 18.8 kDa

Product introduction

Recombinant Human IL-10 Protein is expressed in HEK293 cells without tag. It contains amino acid residues Ser19-Asn178 (UniProt accession: P22301).

Product details

Molecular aliases: Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451
Protein length: Ser19-Asn178
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 18.8 kDa same as Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IL-10 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9ng/ml determined by ELISA (QC Test).

SPR Data — Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).

Cell Based Assay Data — Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL (QC Test).

Background

Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.

References

1.Piazzon MC, Lutfalla G, Forlenza M. IL10, A Tale of an Evolutionarily Conserved Cytokine across Vertebrates. Crit Rev Immunol. 2016;36(2):99‐129. doi:10.1615/CritRevImmunol.2016017480

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Human IL-10 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Based Assay Data

Background

References

Human IL-10 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

SPR

SPR Data

Bioactivity

Cell Based Assay Data

Background

References