Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling)

Name: Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: IL10-CB001

Catalog numberIL10-CB001

HEK293Molecule · IL-10RAMolecular weight · 25.34 kDa

Product introduction

Recombinant Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues His22-Asn233 (UniProt accession: A0A2K5WJ66).

Product details

Molecular aliases: IL-10RA; IL10RA; IL10R; IL-10R1; IL-10R subunit 1; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL10R1; IL10R subunit 1; HIL10R; IL10R alpha
Protein length: His22-Asn233
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 25.34 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus IL-10 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Cynomolgus IL-10 R alpha is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-10 R alpha, His Tag with the EC50 of 49.8ng/ml determined by ELISA.

Background

The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD.

References

1.Mallikarjunappa S, Shandilya UK, Sharma A, Lamers K, Bissonnette N, Karrow NA, Meade KG. Functional analysis of bovine interleukin-10 receptor alpha in response to Mycobacterium avium subsp. paratuberculosis lysate using CRISPR/Cas9. BMC Genet. 2020 Nov 2;21(1):121. doi: 10.1186/s12863-020-00925-4. PMID: 33138773; PMCID: PMC7607837.

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Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling)

Catalog numberIL10-CB001

HEK293Molecule · IL-10RAMolecular weight · 25.34 kDa

Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Cynomolgus IL-10 R alpha Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References