Human IFN gamma R1/IFNGR1 Protein

Name: Human IFN gamma R1/IFNGR1 Protein
Brand: Puweituo Bio
SKU: IFN-HF001

Catalog numberIFN-HF001

HumanHEK293Molecule · IFN-gamma-R1Molecular weight · 52.4 kDa

Product introduction

Recombinant Human IFN gamma R1/IFNGR1 Protein is expressed in HEK293 cells with an hFc (IgG1) tag at the C-terminus. It contains amino acid residues Glu18-Gly245 (accession: AAH05333).

Product details

Molecular aliases: IFN-gamma-R1; CDw119; CD119; IFNGR1; IFNGR; AVP; FLJ45734; IFN-gamma R1; IFN-gR1; type 2; IFNγ; IFNγR1; IFN γR1; IFNγ R1; IFN γ R1; IFNg; IFN gamma; IFN gamma R1; IFN gR1; IFNg R1; IFN g R1
Protein length: Glu18-Gly245
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human IFNGR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human IFN gamma R1 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human IFN gamma, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IFN gamma R1, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.

Background

IFN-gamma and one of its receptor subunits, IFNGR1, are translocated to the nucleus, together with STAT1alpha as one macromolecular complex, via the classical importin-dependent pathway. Put IFN-gamma and its receptor subunit, IFNGR1, in direct contact with the promoter region of IFN-gamma-activated genes with associated increased activity, thus suggesting a transcriptional/cotranscriptional role for IFN-gamma/IFNGR1 as well as a possible role in determining the specificity of IFN-gamma action.

References

1.Ahmed CM, Johnson HM. IFN-gamma and its receptor subunit IFNGR1 are recruited to the IFN-gamma-activated sequence element at the promoter site of IFN-gamma-activated genes: evidence of transactivational activity in IFNGR1. J Immunol. 2006 Jul 1;177(1):315-21. doi: 10.4049/jimmunol.177.1.315. PMID: 16785527.

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Human IFN gamma R1/IFNGR1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Human IFN gamma R1/IFNGR1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References