Biotinylated Human IFN gamma/IFNG Protein

Name: Biotinylated Human IFN gamma/IFNG Protein
Brand: Puweituo Bio
SKU: IFN-HB002

Catalog numberIFN-HB002

HumanHEK293Molecule · Interferon-gammaMolecular weight · 19.1 kDa

Product introduction

Recombinant Biotinylated Human IFN gamma/IFNG Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Gln24-Gly161 (UniProt accession: P01579).

Product details

Molecular aliases: Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ; IFN γ; Interferon-g; IFNg; IFN g
Protein length: Gln24-Gly161
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 85% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS, 8% trehalose (pH 7.4).
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human IFN gamma is greater than 85% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human IFN gamma, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IFN gamma R1, hFc Tag (Cat. IFN-HM2GR) with the EC50 of 3.7ng/ml determined by ELISA.

Background

Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

References

1.Ikeda H, Old LJ, Schreiber RD. The roles of IFN gamma in protection against tumor development and cancer immunoediting. Cytokine Growth Factor Rev. 2002;13(2):95-109. doi:10.1016/s1359-6101(01)00038-7.

加载中…

Biotinylated Human IFN gamma/IFNG Protein

Catalog numberIFN-HB002

HumanHEK293Molecule · Interferon-gammaMolecular weight · 19.1 kDa

Biotinylated Human IFN gamma/IFNG Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human IFN gamma/IFNG Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References