Biotinylated Cynomolgus IFN alpha/beta R2 Protein

Name: Biotinylated Cynomolgus IFN alpha/beta R2 Protein
Brand: Puweituo Bio
SKU: IFN-CB002

Catalog numberIFN-CB002

HEK293Molecule · IFN-R-2Molecular weight · 27.75 kDa

Product introduction

Recombinant Biotinylated Cynomolgus IFN alpha/beta R2 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Ile27-Lys243 (accession: EHH53981.1).

Product details

Molecular aliases: IFN-R-2; IFNAR2; IFNARB; IFNABR; IFN-alpha; IFN-alpha/beta R2; IFNbR2; IFN-R; β receptor 2; β receptor10;; IFN-R2;b receptor 2; beta receptor 2; b receptor10; beta receptor10
Protein length: Ile27-Lys243
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 27.75 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Cynomolgus IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC.

SPR Data — Biotinylated Cynomolgus IFN alpha/beta R2, His-Avi tag captured on CM5 Chip via Anti-His Antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200). (QC Test)

Background

Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well.

References

1.Sehgal M, Zeremski M, Talal AH, Ginwala R, Elrod E, Grakoui A, Li QG, Philip R, Khan ZK, Jain P. IFN-α-Induced Downregulation of miR-221 in Dendritic Cells: Implications for HCV Pathogenesis and Treatment. J Interferon Cytokine Res. 2015 Sep;35(9):698-709. doi: 10.1089/jir.2014.0211. Epub 2015 Jun 19. PMID: 26090579; PMCID: PMC4560851. Copy

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Biotinylated Cynomolgus IFN alpha/beta R2 Protein

Catalog numberIFN-CB002

HEK293Molecule · IFN-R-2Molecular weight · 27.75 kDa

Biotinylated Cynomolgus IFN alpha/beta R2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Background

References

Biotinylated Cynomolgus IFN alpha/beta R2 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

SPR

SPR Data

Background

References