Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer Protein

Name: Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer Protein
Brand: Puweituo Bio
SKU: HPV16E7-HB001

Catalog numberHPV16E7-HB001

HumanHEK293Molecule · HPV16; E7; MHC;Molecular weight · 50.50 kDa

Product introduction

Recombinant Biotinylated Human HPV16 E7(HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119 (B2M) and YMLDLQPET peptide (accession: P04439-1(HLA-A*02:01) & P61769(B2M) & YMLDLQPET peptide).

Product details

Molecular aliases: HPV16; E7; MHC;
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer is greater than 95% as determined by SEC-HPLC.

Background

HPV16 E7 protein, one of the primary target proteins in molecular targeted therapy for HPV-induced cervical cancer. The affitoxin, ZHPV16E7 affitoxin384 was generated by fusing the modified Pseudomonas Exotoxin A (PE38KDEL) to the HPV16 E7-specific affibody.

References

1.Jiang P, Wang L, Hou B, Zhu J, Zhou M, Jiang J, Wang L, Chen S, Zhu S, Chen J, Zhang L. A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer. Theranostics. 2018 Jun 7;8(13):3544-3558. doi: 10.7150/thno.24607. PMID: 30026865; PMCID: PMC6037027.

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Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer Protein

Catalog numberHPV16E7-HB001

HumanHEK293Molecule · HPV16; E7; MHC;Molecular weight · 50.50 kDa

Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References