Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer Protein, Ultra Low Endotoxin

Name: Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer Protein, Ultra Low Endotoxin
Brand: Puweituo Bio
SKU: HLAA2402-HB002

Catalog numberHLAA2402-HB002

HumanHEK293Molecule · MHCMolecular weight · 48.30 kDa

Product introduction

Recombinant Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly25-Thr305(HLA-A*24:02) and Ile21-Met119(B2M) (accession: AAA59600.1(HLA-A*24:02) & P61769(B2M)).

Product details

Molecular aliases: MHC; HLA-A; Peptide Ready
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.01 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA (QC Test).

Background

Peptide Ready HLA-A*24:02&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*24:02. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

References

1.[1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
2.[2] Sun Y, Young MC, Woodward CH, Danon JN, Truong H, Gupta S, Winters TJ, Burslem G, Sgourakis NG. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.

加载中…

Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer Protein, Ultra Low Endotoxin

Catalog numberHLAA2402-HB002

HumanHEK293Molecule · MHCMolecular weight · 48.30 kDa

Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References