Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer Protein, Ultra Low Endotoxin

Name: Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer Protein, Ultra Low Endotoxin
Brand: Puweituo Bio
SKU: HBV-HB004

Catalog numberHBV-HB004

HumanHEK293Molecule · Hepatitis B virusMolecular weight · 50.5 kDa

Product introduction

Recombinant Biotinylated Human HBV(HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-TerminusIt contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and FLLTRILTI peptide (accession: A0A140T913(HLA-A*02:01) & P61769(B2M) & FLLTRILTI peptide).

Product details

Molecular aliases: Hepatitis B virus; MHC; HBV
Protein length: Gly25-Thr305
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 0.01 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer is greater than 95% as determined by SEC-HPLC.

Background

Hepatitis B virus (HBV), is the leading cause of liver diseases infecting an estimated 240 million persons worldwide. The HBV prevalence rates are variables between different countries, with an high level of endemicity in the south-eastern part of Europe. Seven main HBV-D subgenotypes have been described until now (D1-D7).

References

1.Ciccozzi M, Ciccaglione AR, Lo Presti A, Equestre M, Cella E, Ebranati E, Gabanelli E, Villano U, Bruni R, Yalcinkaya T, Tanzi E, Zehender G. Evolutionary dynamics of HBV-D1 genotype epidemic in Turkey. J Med Virol. 2014 Jan;86(1):109-16. doi: 10.1002/jmv.23740. Epub 2013 Oct 24. PMID: 24243521.

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Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer Protein, Ultra Low Endotoxin

Catalog numberHBV-HB004

HumanHEK293Molecule · Hepatitis B virusMolecular weight · 50.5 kDa

Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human HLA-A*02:01&B2M&HBV (FLLTRILTI) Monomer Protein, Ultra Low Endotoxin

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References