Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein

Name: Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein
Brand: Puweituo Bio
SKU: H2KB-HB003

Catalog numberH2KB-HB003

MouseHEK293Molecule · MHCMolecular weight · 48.3 kDa

Product introduction

Recombinant Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains His24-Pro297 (H-2K(b)) and Ile21-Met119(B2M) (accession: P01901(H-2K(b)) & P01887(B2M)).

Product details

Molecular aliases: MHC; H-2K(b); Peptide Ready
Protein length: His24-Pro297
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 100mM L-arginine (pH 7.4).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer is greater than 95% as determined by SEC-HPLC.

Background

H-2K(b)&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching H-2K(b). Peptide ready MHC molecules comprising mouse H-2K(b) alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

References

1.[1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
2.[2] Sun Y, Young MC, Woodward CH, Danon JN, Truong H, Gupta S, Winters TJ, Burslem G, Sgourakis NG. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.

加载中…

Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein

Catalog numberH2KB-HB003

MouseHEK293Molecule · MHCMolecular weight · 48.3 kDa

Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References