Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein

Name: Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein
Brand: Puweituo Bio
SKU: GFRAL-CB001

Catalog numberGFRAL-CB001

HEK293Molecule · GFR alpha-likeMolecular weight · 40.7 kDa

Product introduction

Recombinant Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein is expressed in HEK293 cells with a His tag and Avi tag at the N-terminus. It contains amino acid residues Gln20-Glu351 (accession: XP_015304775.2).

Product details

Molecular aliases: GFR alpha-like; GFRAL; GRAL; C6orf144
Protein length: Gln20-Glu351
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22μm filtered solution in 20mM PB, 400mM NaCl (pH 6.0).
Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — Biotinylated Cynomolgus GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Cynomolgus GFRAL is greater than 90% as determined by SEC-HPLC.

ELISA Data — Immobilized Cynomolgus GDF15, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 39.9ng/ml determined by ELISA (QC Test).

Background

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains amino acid residues a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

References

1.Mullican S E, Lin-Schmidt X, Chin C N, et al. GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates[J]. Nature Medicine, 2017, 23(10):1150-1157.

加载中…

Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein

Catalog numberGFRAL-CB001

HEK293Molecule · GFR alpha-likeMolecular weight · 40.7 kDa

Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References