Biotinylated Mouse GDF15 Protein (Primary Amine Labeling)

Name: Biotinylated Mouse GDF15 Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: GDF15-MB001

Catalog numberGDF15-MB001

MouseHEK293Molecule · GDF-15Molecular weight · 37.9 kDa

Product introduction

Recombinant Biotinylated Mouse GDF15 Protein (Primary Amine Labeling) is expressed in HEK293 cells with an hFc (IgG1) tag at the N-terminus. It contains amino acid residues Ser189-Ala303 (UniProt accession: Q9Z0J7).

Product details

Molecular aliases: GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β; NAG1; RG1; Placental TGF-b; PTGF-b; PTGFBPTGF-b; Growth differentiation factor-15; Growth differentiation factor 15
Protein length: Ser189-Ala303
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Mouse GDF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Mouse GDF15 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Mouse GFRAL, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GDF15, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. (QC Test)

Background

Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.

References

1.Luan HH, Wang A, Hilliard BK, Carvalho F, Rosen CE, Ahasic AM, Herzog EL, Kang I, Pisani MA, Yu S, Zhang C, Ring AM, Young LH, Medzhitov R. GDF15 Is an Inflammation-Induced Central Mediator of Tissue Tolerance. Cell. 2019 Aug 22;178(5):1231-1244.e11. doi: 10.1016/j.cell.2019.07.033. Epub 2019 Aug 8. PMID: 31402172; PMCID: PMC6863354. Copy

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Biotinylated Mouse GDF15 Protein (Primary Amine Labeling)

Catalog numberGDF15-MB001

MouseHEK293Molecule · GDF-15Molecular weight · 37.9 kDa

Biotinylated Mouse GDF15 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Biotinylated Mouse GDF15 Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References