Biotinylated Human GCGR/Glucagon receptor Nanodisc

Name: Biotinylated Human GCGR/Glucagon receptor Nanodisc
Brand: Puweituo Bio
SKU: GCGR-HB003

Catalog numberGCGR-HB003

HumanHEK293Molecule · Glucagon receptorMolecular weight · 66.9 kDa

Product introduction

Recombinant Biotinylated Human GCGR/Glucagon receptor Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Phe477 (UniProt accession: P47871).

Product details

Molecular aliases: Glucagon receptor; GL-R; GCGR; Glucagon R
Protein length: Met1-Phe477
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 66.9 kDa.
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Negative control: CON-HM1NB

Data display

ELISA Data — Immobilized Biotinylated Human GCGR Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GCGR Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test).

SPR Data — Biotinylated Human GCGR Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GCGR Antibody, hFc Tag with an affinity constant of 5.02 nM as determined in SPR assay (Biacore T200).

BLI Data — Loaded Biotinylated Human GCGR Nanodisc, His Tag on Streptavidin -Biosensor can bind Anti-GCGR Antibody, hFc Tag with an affinity constant of 8.61 nM as determined in BLI assay.

Background

The pancreatic hormone glucagon activates the glucagon receptor (GCGR), a class B seven-transmembrane G protein-coupled receptor that couples to the stimulatory heterotrimeric G protein and provokes PKA-dependent signaling cascades vital to hepatic glucose metabolism and islet insulin secretion. The GCGR expressed at the plasma membrane is constitutively ubiquitinated and upon agonist-activation, internalized GCGRs are deubiquitinated at early endosomes and recycled via Rab4-containing vesicles.

References

1.Kaur S, Sokrat B, Capozzi ME, El K, Bai Y, Jazic A, Han B, Krishna Kumar K, D'Alessio DA, Campbell JE, Bouvier M, Shenoy SK. The ubiquitination status of the glucagon receptor determines signal bias. J Biol Chem. 2023 May;299(5):104690. doi: 10.1016/j.jbc.2023.104690. Epub 2023 Apr 8. PMID: 37037304; PMCID: PMC10200972.

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Biotinylated Human GCGR/Glucagon receptor Nanodisc

Catalog numberGCGR-HB003

HumanHEK293Molecule · Glucagon receptorMolecular weight · 66.9 kDa

Product introduction

Recombinant Biotinylated Human GCGR/Glucagon receptor Nanodisc is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Met1-Phe477 (UniProt accession: P47871).

Biotinylated Human GCGR/Glucagon receptor Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References

Biotinylated Human GCGR/Glucagon receptor Nanodisc

Product introduction

Product details

Data display

ELISA

ELISA Data

SPR

SPR Data

BLI

BLI Data

Background

References