Non-biotinylated Human FOLR1 Protein

Name: Non-biotinylated Human FOLR1 Protein
Brand: Puweituo Bio
SKU: FOLR1-HP001

Catalog numberFOLR1-HP001

HumanHEK293Molecule · FR-alphaMolecular weight · 27.5 kDa

Product introduction

Recombinant Human FOLR1 Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Arg25-Met233 (UniProt accession: P15328).

Product details

Molecular aliases: FR-alpha; FR alpha; FR α; FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18; Folate receptor 1; Fbp1; Folate receptor alpha;
Protein length: Arg25-Met233
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Human FOLR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Human FOLR1 is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized Human FOLR1, His Tag at 1 μg/ml (100 μl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.6 ng/ml determined by ELISA (QC Test).

Background

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.

References

1.Anthony C, Bax H J, Josephs D H, et al. Targeting folate receptor alpha for cancer treatment[J]. Oncotarget, 2016, 7(32).

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Non-biotinylated Human FOLR1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References

Non-biotinylated Human FOLR1 Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Background

References