Biotinylated Human Fibronectin (32-345) Protein (Primary Amine Labeling)

Name: Biotinylated Human Fibronectin (32-345) Protein (Primary Amine Labeling)
Brand: Puweituo Bio
SKU: FIBRONECTIN-HB001

Catalog numberFIBRONECTIN-HB001

HumanHEK293Molecule · FNMolecular weight · 35.93 kDa

Product introduction

Recombinant Biotinylated Human Fibronectin (32-345) Protein (Primary Amine Labeling) is expressed in HEK293 cells with a His tag at the C-terminus. It contains amino acid residues Gln32-Thr345 (UniProt accession: P02751-1).

Product details

Molecular aliases: FN; CIG; FN1; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF; SMDCF
Protein length: Gln32-Thr345
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 35.93 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
Endotoxin: Less than 1 EU per μg by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
State: Lyophilized
Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Reconstitution: Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

Data display

Bis-Tris PAGE Data — Biotinylated Human Fibronectin (32-345) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of Biotinylated Human Fibronectin (32-345) is greater than 90% as determined by SEC-HPLC.

Background

Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans.

References

1.Ruoslahti E. Fibronectin. J Oral Pathol. 1981 Feb;10(1):3-13. doi: 10.1111/j.1600-0714.1981.tb01242.x. PMID: 6782207.

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Biotinylated Human Fibronectin (32-345) Protein (Primary Amine Labeling)

Catalog numberFIBRONECTIN-HB001

HumanHEK293Molecule · FNMolecular weight · 35.93 kDa

Biotinylated Human Fibronectin (32-345) Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References

Biotinylated Human Fibronectin (32-345) Protein (Primary Amine Labeling)

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

Background

References