FITC-Labeled Human EGFRVIII Protein

Name: FITC-Labeled Human EGFRVIII Protein
Brand: Puweituo Bio
SKU: EGFRVIII-HP001

Catalog numberEGFRVIII-HP001

HumanHEK293Molecule · ErbBMolecular weight · 41.6 kDa

Product introduction

Recombinant FITC-Labeled Human EGFRVIII Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu25-Ser378 (accession: NP_001333870.1).

Product details

Molecular aliases: ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Protein length: Leu25-Ser378
Expression system: HEK293
Molecular weight: The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Formulation: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Storage: Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Data display

Bis-Tris PAGE Data — FITC-Labeled Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC Data — The purity of FITC-Labeled Human EGFRVIII is greater than 95% as determined by SEC-HPLC.

ELISA Data — Immobilized FITC-Labeled Human EGFRVIII, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test).

Flow Cytometry Data — FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5μg/ml FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

Background

The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.

References

1.Pedersen M W, Meltorn M, Damstrup L, et al. The type III epidermal growth factor receptor mutation Biological significance and potential target for anti-cancer therapy[J]. Annals of Oncology, 2001, 12(6):745-760.

加载中…

FITC-Labeled Human EGFRVIII Protein

Catalog numberEGFRVIII-HP001

HumanHEK293Molecule · ErbBMolecular weight · 41.6 kDa

Product introduction

Recombinant FITC-Labeled Human EGFRVIII Protein is expressed in HEK293 cells with a His tag and Avi tag at the C-terminus. It contains amino acid residues Leu25-Ser378 (accession: NP_001333870.1).

FITC-Labeled Human EGFRVIII Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Flow Cytometry

Flow Cytometry Data

Background

References

FITC-Labeled Human EGFRVIII Protein

Product introduction

Product details

Data display

Bis-Tris PAGE

Bis-Tris PAGE Data

SEC-HPLC

SEC-HPLC Data

ELISA

ELISA Data

Flow Cytometry

Flow Cytometry Data

Background

References